Journal article
Role of lysophosphatidic acid in the retinal pigment epithelium and photoreceptors
GE Lidgerwood, AJ Morris, A Conquest, M Daniszewski, LA Rooney, SY Lim, D Hernández, HH Liang, P Allen, PP Connell, RH Guymer, AW Hewitt, A Pébay
Biochimica Et Biophysica Acta Molecular and Cell Biology of Lipids | ELSEVIER SCIENCE BV | Published : 2018
Abstract
The human retina is a complex structure of organised layers of specialised cells that support the transmission of light signals to the visual cortex. The outermost layer of the retina, the retinal pigment epithelium (RPE), forms part of the blood retina barrier and is implicated in many retinal diseases. Lysophosphatidic acid (LPA) is a bioactive lipid exerting pleiotropic effects in various cell types, during development, normal physiology and disease. Its producing enzyme, AUTOTAXIN (ATX), is highly expressed by the pigmented epithelia of the human eye, including the RPE. Using human pluripotent stem cell (hPSC)-derived retinal cells, we interrogated the role of LPA in the human RPE and ph..
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Grants
Awarded by University of Melbourne
Funding Acknowledgements
The authors declare no conflict of interest. We thank J. Thomson (University of Wisconsin) for providing the iPSC line iPS (Foreskin) 4 clone 2; I. Trounce (Centre for Eye Research Australia) and M. Al-Ubaidi (University of Oklahoma, USA) for providing the immortalized mouse 661W line; Penny A. Mckelvie (St Vincent Hospital, Melbourne, Australia) for sectioning of the human eyes, Annie K. Mcauley (CERA) and Tejal Kulkarni (CERA) for technical assistance, the Lions Eye Donation Service (Melbourne) for providing human donor eyes, Alastair G. Stewart (University of Melbourne) for providing access to the EVOM2 voltohemeter, and the Melbourne Brain Centre's flow Cytometry core facility. This work was supported by grants from the National Health and Medical Research Council (NHMRC, 1059369), The Ophthalmic Research Institute of Australia, Retina Australia, the Joan and Peter Clemenger Foundation, the Philip Neal bequest, the Centre for Stem Cell Systems, the JEM Research Foundation, the Centre for Eye Research Australia Foundation, a NHMRC Dora Lush Biomedical Postgraduate Research Scholarship 1133237 (AC), a NHMRC Research Fellowship 1103013 (RHG), a NHMRC Practitioner Fellowship 1103329 (AWH), an Australian Research Council Future Fellowship FT140100047 (AP), Australian Postgraduate Award Scholarships (GEL, MD), an International Postgraduate Award Scholarships (MD), the University of Melbourne and Operational Infrastructure Support from the Victorian Government.